I believe it was you in this
post that suggested the 8616SX only supports fame sizes up to 1920?
Are you saying that the same setup below works properly if the source and destination are in the same VLAN, but it doesn't work properly if the source and destination are in different VLANs and the 8600 must routing the packet as opposed to just switching it?
I'd be suspect of the hardware... what version of software are you running on the 8600?
I'm assuming you have upgraded the 5500 series switches to 6.0/6.1? I can't remember which version of software is required for Jumbo Frame support right now.
Cheers!